Translational_Unit

Part:BBa_K4002007:Design

Designed by: Nixue Song   Group: iGEM21_Xiamen_City   (2021-10-12)


Cas9+gRNA+HR-L-endo-pgaA-HR-R


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4971
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 688
    Illegal BglII site found at 1625
    Illegal BglII site found at 6156
    Illegal BglII site found at 6714
    Illegal BamHI site found at 2443
    Illegal XhoI site found at 3981
    Illegal XhoI site found at 4584
    Illegal XhoI site found at 6674
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3177
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 6413
    Illegal BsaI.rc site found at 5915
    Illegal BsaI.rc site found at 6785


Design Notes

No


Source

No

References